Here are the commands to execute each of the funtions once the package in installed:
library(MyFirstPackage)
hspc <- ReadNewSCell("Mouse_HSPC_reduced_v2.txt") hspc <- GetVarGenes(hspc,150) hspc <- readFACS(hspc,"Mouse_HSPC_reduced_IndexSortData.txt") MakeHeatMap(hspc)
hspc <- CalcTSNE(hspc)
hspc <- clusterCells(hspc,5) #make 5 clusters of cells ViolinPlotFACSfromCluster(hspc,1) # violin plots of FACS data for cluster 1 ColourTSNEbyClust(hspc) ColourTSNEbyGeneExpr(hspc,"Mpo") # colour by expression of Mpo