ReadPlate is an ImageJ macro by José María Delfino for measuring light intensity and calculating uncorrected, blank, and blank-corrected absorbance values from a color photograph of a multi-well plate. Version 3.0 supports 6-, 12-, 24-, 48-, and 96-well plates.
ReadPlate is registered in ImageJ's canonical update-site list as ReadPlate, but the registry's original sites.imagej.net endpoint was not reachable during this release audit. A working, updater-compatible site is now published at:
https://ghanerka.github.io/ReadPlate/
Until the canonical URL update is merged:
- Start a current Fiji installation and choose Help › Update….
- Click Manage update sites, then Add Unlisted Site.
- Use a nickname such as
ReadPlate-GitHuband URLhttps://ghanerka.github.io/ReadPlate/. - Enable the site, click Apply and Close, apply changes, and restart Fiji.
- Run Plugins › ReadPlate 3.0.
The updater installs the macro, original HTML documentation, and original JPEG sample image. After registry PR #217 is merged, users can simply enable the built-in ReadPlate entry instead of adding the URL.
The updater belongs to ImageJ2/Fiji; the original ImageJ1 application by itself does not support update sites. For a manual ImageJ1 installation, open ImageJ and choose Plugins › Install…, then select ReadPlate3.0.txt.
- An RGB plate image (the macro exits for non-RGB images).
- ImageJ 1.43h or newer (
requires("1.43h")). - Version 3.0 was tested by its author with ImageJ 1.53c and Java 8.
Before running ReadPlate, use Analyze › Set Measurements… and enable:
- Area
- Standard deviation
- Min & max gray value
- Mean gray value
- Modal gray value
- Add to overlay
- Redirect to: None
- Decimal places: 3
See index.html for the original release documentation and plate.jpg for the original sample plate image.
The source release preserves the supplied 2020 artifacts byte-for-byte:
| File | Purpose |
|---|---|
ReadPlate3.0.txt |
ImageJ macro source, version 3.0 |
index.html |
Original documentation |
plate.jpg |
Original 96-well sample image |
imagej/ij.jar |
Local ImageJ 1.53c runtime; retained in the local bundle but intentionally excluded from source control and update-site payloads |
The bundled runtime is not needed when installing through Fiji. Checksums and provenance are recorded in release-manifest.json.
- Version: 3.0
- Release date: 2020-08-15
- Author: José María Delfino
- Relevant publication: Angelani et al. (2018), “A Metabolic Control Analysis Approach to Introduce the Study of Systems in Biochemistry: the Glycolytic Pathway in the Red Blood Cell,” https://doi.org/10.1002/bmb.21139
Machine-readable citation metadata is in CITATION.cff.
The supplied release bundle contains no software license declaration. See LICENSE.md; no open-source license has been inferred or added.
ImageJ's central update-site registry has contained a ReadPlate entry since 2020. The original entry was merged as PR #54; registry PR #217 points that entry to the verified working site, and wiki PR #433 adds the missing ImageJ plugin page. Both upstream PRs are open. See DISTRIBUTION.md for exact discovery, hosting, and clean-install verification states.