Protocols

Protocols for the lab

As we work mainly with Petunia hybrida, protocals are optimized accordingly.

Name		Name	Last commit message	Last commit date
Github		Github
Anthocyanin_quantification		Anthocyanin_quantification
CRISPR_Cas_constructs.md		CRISPR_Cas_constructs.md
DNA_isolation_from_Yeast.md		DNA_isolation_from_Yeast.md
First_strand_cDNA_synthesis.md		First_strand_cDNA_synthesis.md
Gateway_Cloning.md		Gateway_Cloning.md
In_Situ_Hybridisation.md		In_Situ_Hybridisation.md
PCR.md		PCR.md
PREPARATION OF PETAL PROTOPLASTS.md		PREPARATION OF PETAL PROTOPLASTS.md
PREPARATION_OF_CELLS_COMPETENT_FOR_ELECTROPORATION		PREPARATION_OF_CELLS_COMPETENT_FOR_ELECTROPORATION
Petunia_DNA.md		Petunia_DNA.md
Petunia_Transformation_Protocol.md		Petunia_Transformation_Protocol.md
README.md		README.md
RNA_isolation_(trizol).md		RNA_isolation_(trizol).md
ROS_detection_in_protoplasts.md		ROS_detection_in_protoplasts.md
Seed Sterilization		Seed Sterilization
Sowing_on_agar.md		Sowing_on_agar.md
Thin Layer Chromatography (TLC) analysis of anthocyanins.md		Thin Layer Chromatography (TLC) analysis of anthocyanins.md
X-Gal_Agarose_Overlay_Assay.md		X-Gal_Agarose_Overlay_Assay.md
Yeast_Transformation.md		Yeast_Transformation.md
isolation microsomal fraction.md		isolation microsomal fraction.md
vacuole isolation petunia flower.md		vacuole isolation petunia flower.md
vacuolino isolation.md		vacuolino isolation.md

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