@@ -12,21 +12,22 @@ Install
1212
1313To install ChIP-R:
1414
15- git clone git@github.com : rhysnewell /ChIP-R.git
16-
17- We recommend you add the src folder to your pythonpath variable in your .bashrc profile
15+ git clone https://github.com/rhysnewell/ChIP-R.git
16+ cd ChIP-R
17+ python3 setup.py install
18+
1819
1920
2021Usage
2122-----
2223
23- In the command line, type in ** 'python3 ChIP-R.py -h '** for detailed usage.
24+ In the command line, type in ** 'chipr -h '** for detailed usage.
2425
25- $ python3 ChIP-R.py -h
26+ $ chipr -h
2627
27- usage: ChIP-R [-h] -i INPUT [INPUT ...] [-o OUTPUT] [-m MINENTRIES]
28- [--rankmethod RANKMETHOD] [--duphandling DUPHANDLING]
29- [--seed RANDOM_SEED] [-a ALPHA]
28+ usage: chipr [-h] -i INPUT [INPUT ...] [-o OUTPUT] [-m MINENTRIES]
29+ [--rankmethod RANKMETHOD] [--duphandling DUPHANDLING]
30+ [--seed RANDOM_SEED] [-a ALPHA]
3031
3132 Combine multiple ChIP-seq files and return a union of all peak locations and a
3233 set confident, reproducible peaks as determined by rank product analysis
@@ -36,9 +37,9 @@ In the command line, type in **'python3 ChIP-R.py -h '** for detailed usage.
3637 -i INPUT [INPUT ...], --input INPUT [INPUT ...]
3738 ChIP-seq input files. These files must be in either
3839 narrowPeak, broadPeak, or regionPeak format. Multiple
39- inputs a separeted by a single space
40+ inputs are separeted by a single space
4041 -o OUTPUT, --output OUTPUT
41- ChIP-seq output filename
42+ ChIP-seq output filename prefix
4243 -m MINENTRIES, --minentries MINENTRIES
4344 The minimum peaks between replicates required to form
4445 an intersection of the peaks Default: 1
@@ -54,17 +55,18 @@ In the command line, type in **'python3 ChIP-R.py -h '** for detailed usage.
5455 'average'
5556 --seed RANDOM_SEED Specify a seed to be used in conjunction with the
5657 'random' option for -duphandling Must be between 0 and
57- 1 default : 0.5
58+ 1 Default : 0.5
5859 -a ALPHA, --alpha ALPHA
5960 Alpha specifies the user cut-off value for set of
6061 reproducible peaks The analysis will still produce
6162 results including peaks within the threshold
62- calculatedusing the binomial method default: 0.05
63+ calculatedusing the binomial method Default: 0.05
64+
6365
6466
6567Example
6668------
67- $ python3 ChIP-R.py -i input_prefix1.bed input_prefix2.bed input_prefix3.bed input_prefix4.bed -m 2 -o output_prefix
69+ $ chipr -i input_prefix1.bed input_prefix2.bed input_prefix3.bed input_prefix4.bed -m 2 -o output_prefix
6870
6971Output
7072------
@@ -93,6 +95,6 @@ Citation
9395Contact
9496-------
9597
96- Authors: Rhys Newell, Alexandra Essebier, Michael Piper, Mikael Boden
98+ Authors: Rhys Newell, Michael Piper, Mikael Boden, Alexandra Essebier
9799
98100Contact: rhys.newell(AT)uq.net.au
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