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PathoNiche: Persistent HSC Scar Topology

DOI

Analysis-code snapshots, non-identifying aggregate clinical results, public-data-derived molecular and spatial results, and publication figures supporting the manuscript “Persistent stellate-cell programs form larger connected scar regions with fewer repair interfaces in advanced human liver fibrosis.”

Submission-aligned release: v1.2.0
Repository: https://github.com/LightChainr/PathoNiche-ResolverWindow
Archived release: https://doi.org/10.5281/zenodo.22138229

Scientific scope

The repository supports five principal findings:

  1. HSC-retention coupling to the scar field strengthens across human fibrosis stages.
  2. HSC-dominant regions enlarge and become more spatially connected as fibrosis advances.
  3. Interfaces between HSC-dominant tissue and endothelial/macrophage repair-associated regions contract with fibrosis stage.
  4. Injury withdrawal produces an incomplete molecular return toward the control state.
  5. Multi-omic and perturbation analyses nominate regulators and treatment-sequence experiments for direct testing.

The clinical component contains only non-identifying aggregate tables from an 8,142-patient index cohort and a 772-patient longitudinal elastography cohort. No participant-level clinical record is distributed.

Archived release

The versioned research companion, including analysis-code snapshots, derived aggregate outputs, documentation, sensitivity results, and publication figures, is archived on Zenodo:

Qiao C, Ying H, Bu L, Xiong W. Persistent HSC Scar Topology: Analysis Code and Derived Data for Human Liver Fibrosis. Version 1.2.0. Zenodo; 2026. https://doi.org/10.5281/zenodo.22138229

The GitHub repository provides the public development and issue-tracking location. The Zenodo record is the archival citation for the submission-aligned release.

Reproducibility levels

Level Contents Publicly runnable
A Verification of key reported numerical results Yes, from the archived release
B Inspection of derived UQ, mouse, LINCS, and aggregate clinical results Yes, from included files
C Full reconstruction of public molecular and spatial analyses Yes, after obtaining the public raw data listed in the archive
D Patient-level clinical and flow analyses No; these require institutional approval and governed source data

Data governance

Excluded material includes participant-level records, dates linked to individuals, direct or transformed clinical identifiers, linkage keys, ethics documents, author contact files, credentials, raw third-party sequencing or imaging archives, model weights, and manuscript drafts.

Licenses

  • Original analysis code: MIT License.
  • Author-owned documentation, figures, and derived tabular outputs: Creative Commons Attribution 4.0 International.
  • Third-party source datasets remain governed by their original terms and citation requirements.

Machine-readable citation metadata are provided in CITATION.cff and CITATION.bib.