README.md

Multi-FASTA-Splitter

A lightweight fast windows gui app that splits a multi fasta file to individual single fasta files

Citations

1. Release v475 Genome Browser Software · ucscGenomeBrowser/kent. Retrieved 7 February 2025, from https://github.com/ucscGenomeBrowser/kent/releases/tag/v475_branch.1
2. Tsiouri Olga. Multi FASTA Splitter: A lightweight fast windows gui app that splits a multi fasta file to individual single fasta files.https://github.com/BioGUIwsl1Lab/Multi-FASTA-Splitter

Dependencies

1. Windows 10 version 2004 and higher (Build 19041 and higher) or Windows 11(type winver on the search button and click winver to find your OS version)
2. Windows Subsystem for linux(wsl2)
3. the file Multi FASTA Splitter.exe in the bin folder

Usage

1. Click on the Multi FASTA Splitter.exe file

2. Click browse and select an input file

3. Click Run program

As seen in the folder the program creates an output folder to store the single-FASTA files

if you want to reformat the output fasta files download and open Single FASTA Formatter.exe from the bin folder:

1. Click browse and select the directory where the output .fa files are located.
2. Select the FASTA Width
3. Click Run program

The example input/output files can be seen on the data folder

Input date were retrieved from solgenomics.net tomato annotation version 4.1.